Bioinformatics Tutorials

Perl Script to Split FASTA Files A simple guide to efficiently splitting large genome files using Perl. Extract Matching Data from Two Files Using Python Learn how to cross-reference two large datasets and extract matches. Variant Annotation Pipeline Tutorial A step-by-step pipeline using SnpEff and ANNOVAR for variant annotation. Using BWA to Align Sequenced Reads Align raw sequencing reads to a reference genome using the Burrows-Wheeler Aligner. Calculate GC Content with Python A simple Python tool to calculate GC content directly from sequence data. SRA-toolkit Guide Using SRA to download and process sequencing data from the NCBI database. Extracting Variants Using SnpSift Techniques for filtering and extracting specific variants from VCF files. Convert VCF to HapMap Format A guide on converting variant call files (VCF) to HapMap format for GWAS. Analysis of VCF File Tutorial Comprehensive methods for analyzing variant call files and extracting statistics. Writing a Bash Script Introduction to writing Bash scripts for bioinformatics automation. Population Genetic Analysis A tutorial of population genetic analysis techniques.