Perl Script to Split FASTA Files

How to Use This Script

This tutorial provides a Perl script for splitting a FASTA file into multiple output files.

Steps:

1. Download this script and save it to your current working directory.
2. Open a terminal or command prompt.
3. Navigate to the directory where the script fasta_split.pl is saved.

Executing the Script:

To split a FASTA file into multiple output files, run the following command:

perl fasta_split.pl -i input_file.fasta -o output_prefix -n num_files
        

Parameters:

• -i input_file.fasta: Replace this with the path to your input FASTA file.
• -o output_prefix: Specify the desired prefix for the output files. For example, if you use output, the script will generate files named output-split_1.fasta, output-split_2.fasta, and so on.
• -n num_files: Set this to the desired number of output files.

Script Output:

After executing the command, the script will create a directory named split_files (if it doesn't already exist) in the current working directory. Inside this directory, you will find the split FASTA files generated by the script.

Important Notes:

• This script may not be suitable for very large FASTA files.
• Ensure that you have Perl installed on your system before running the script.